Restituting intestinal epithelial cells exhibit increased transducibility by adenoviral vectors
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چکیده
منابع مشابه
Differentiated intestinal epithelial cells exhibit increased migration through polyamines and myosin II.
Early mucosal restitution is a rapid process by which differentiated intestinal epithelial cells migrate to reseal superficial wounds. However, most of the in vitro studies for restitution employ undifferentiated intestinal crypt cells as a model. The transcription factor, Cdx2, plays an important role in the regulation of intestinal epithelial differentiation. Forced expression of the Cdx2 gen...
متن کاملTargeted delivery of adenoviral vectors by cytotoxic T cells.
Effective targeting of vectors to tumor cells that have metastasized to multiple different tissue sites remains a major challenge for gene therapy. Tumor-specific cytotoxic T lymphocytes (CTLs) have been shown in animal models and in humans to be able to cross tissue barriers and traffic to tumor cells. However, their capacity to eliminate malignancy has been limited by tumor immune evasion str...
متن کاملDifferentiated intestinal epithelial cells express high levels of TGF-β receptors and exhibit increased sensitivity to growth inhibition.
BACKGROUND Intestinal epithelial cells (IECs) within crypts continuously divide and differentiate as they migrate up towards the luminal surface of the mucosa. With the onset of differentiation, IECs lose their proliferative potential, but the exact mechanism remains unknown. This current study examined the involvement of the TGF-β signaling pathway in this process. METHODS Studies were condu...
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Intestinal epithelial cells secrete a protective luminal mucus barrier inhibiting viral gene transfer. Quiescent, polarized monolayers of primary epithelial cells from dog gallbladder and human colon are efficiently transduced through the apical mucus side by lentivirus vectors, suggesting their application to intestinal gene therapy.
متن کاملImproved gene delivery to intestinal mucosa by adenoviral vectors bearing subgroup B and d fibers.
A major obstacle to successful oral vaccination is the lack of antigen delivery systems that are both safe and highly efficient. Conventional replication-incompetent adenoviral vectors, derived from human adenoviruses of subgroup C, are poorly efficient in delivering genetic material to differentiated intestinal epithelia. To date, 51 human adenovirus serotypes have been identified and shown to...
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ژورنال
عنوان ژورنال: The Journal of Gene Medicine
سال: 2006
ISSN: 1099-498X,1521-2254
DOI: 10.1002/jgm.981